研究成果

•Chlorophyll fluorescence responses to CO2 availability reveal crassulacean acid metabolism in epiphytic orchids
Bekki S, Suetsugu K, Kobayashi K (2025) J Plant Res, 138, 323–336. 
https://doi.org/10.1007/s10265-024-01608-2

•Photosynthetic capacity and pigment distribution of a siphonous green alga, Dichotomosiphon tuberosus
Seki S, Kobayashi K, Fujii R (2025) Photosynth Res, accepted.

•Rapid development of naked malting barley germplasm through targeted mutagenesis
Hisano H, Sakai H, Hamaoka M, Munemori H, Abe F, Meints B, Sato K, Hayes PM (2025) Rapid development of naked malting barley germplasm through targeted mutagenesis. Molecular Breeding 45:32,
https://doi.org/10.1007/s11032-025-01553-5

•Distinct Infection Mechanisms of Rhizoctonia solani AG-1 IA and AG-4 HG-I+II in Brachypodium distachyon and Barley
Mahadevan, N., Fernanda, R., Kouzai, Y., Kohno, N., Nagao, R., Nyein, K. T., Watanabe, M., Sakata, N., Matsui, H., Toyoda, K., Ichinose, Y., Mochida, K., Hisano, H., & Noutoshi, Y. (2025). Distinct Infection Mechanisms of Rhizoctonia solani AG-1 IA and AG-4 HG-I+II in Brachypodium distachyon and Barley. Life, 15, 235. https://doi.org/10.3390/life15020235

•Arabidopsis thaliana ACTIN DEPOLYMERIZING FACTORs play a role in leaf senescence regulation
Matsumoto T, Kobayashi K, Inada N bioRxiv, 2024.05. 14.594232.

•Mutations in starch BRANCHING ENZYME 2a suppress the traits caused by the loss of ISOAMYLASE1 in barley
Matsushima, R., Hisano, H., Kim, JS. McNelly R, Oitome NF, Seung D, Fujita N, Sato K. (2024) Mutations in starch BRANCHING ENZYME 2a suppress the traits caused by the loss of ISOAMYLASE1 in barley. Theor Appl Genet 137, 212. https://doi.org/10.1007/s00122-024-04725-7

•Possible roles of immunity-related response in modulating chlorosis induced by the silencing of chloroplast HSP90C in tobacco models.
Unung, O.O., Bensedira, H.E.S., Matsuura, T., Mori, I.C., Shimomura, Y., Yaeno, T., Kaya, H., Kobayashi, K.
Journal of General Plant Pathology (2024). https://doi.org/10.1007/s10327-024-01191-3

•CRISPR/Cas9-mediated resurrection of tobacco NB-LRR class virus resistance gene from a susceptible allele with partial duplication.
Miyoshi, S., Unung, O.O., Kaya, H., Yaeno, T., Kobayashi, K.
Journal of General Plant Pathology (2024). https://doi.org/10.1007/s10327-024-01189-x

•Comprehensive survey of copper resistance and analysis of responsible genes in Pseudomonas syringae pv. actinidiae biovar 1 and biovar 3 isolates from Japan.
Aono, M., Miyoshi, T., Yagi, H., Shimizu, S., Shinozaki, T., Yaeno, T., Kobayashi, K.
Journal of General Plant Pathology 90, 134–143 (2024). https://doi.org/10.1007/s10327-024-01169-1

•Membrane association of active genes organizes the chloroplast nucleoid structure
VM Palomar, Y Cho, S Fujii, MH Rothi, S Jaksich, J-H Min, AN Schlachter, J Wang, Z Liu, AT Wierzbicki (2024) Proceedings of the National Academy of Sciences 121 (28): e2309244121

•Orchestration of Photosynthesis-Associated Gene Expression and Galactolipid Biosynthesis during Chloroplast Differentiation in Plants
S Fujii*, H Wada, K Kobayashi (2024)  Plant and Cell Physiology, 65 (6): 1014–1028

•Mechanisms for introducing 250 kDa fluorescent molecules and Cas9/sgRNA into plant cells by plasma treatment.
Ikeda, Y., Hamada, Y., Ueshima, R., Kido, Y., Yaeno, T., Kaya, H., Kobayashi, K., Jinno, M.
Japanese Journal of Applied Physics. 62, SL1015 (2023). https://doi.org/10.35848/1347-4065/acd3f9

•Biosynthesis of phosphatidylglycerol in photosynthetic organisms.
Kobayashi K, Jimbo H, Nakamura Y, Wada H.
Prog. Lipid Res. 93:101266, 2023 https://doi.org/10.1016/j.plipres.2023.101266

•Anionic lipids facilitate membrane development and protochlorophyllide biosynthesis in etioplasts.
Yoshihara A,  Kobayashi K, Nagata N, Fujii S, Wada H, Kobayashi K.
Plant Physiol. 194(3): 1692-1704, 2023. https://doi.org/10.1093/plphys/kiad604

•Evolutionary implications from lipids in membrane bilayers and photosynthetic complexes in cyanobacteria and chloroplasts.
Kobayashi K, Yoshihara A, Kubota-Kawai H.
J. Biochem. 174(5):399-408. https://doi.org/10.1093/jb/mvad058

• A cell wall–localized cytokinin/purine riboside nucleosidase is involved in apoplastic cytokinin metabolism in Oryza sativa.
Kojima M, Makita N, Miyata K, Yoshino M, Iwase A, Ohashi M, Surjana A, Kudo T, Takeda-Kamiya N, Toyooka K, Miyao A, Hirochika H, Ando T, Shomura A, Yano M, Yamamoto T, Hobo T, Sakakibara H
Proceedings of the National Academy of Sciences 120: e2217708120, 2023.　https://www.pnas.org/doi/full/10.1073/pnas.2217708120
 
• WUSCHEL-RELATED HOMEOBOX 13 suppresses de novo shoot regeneration via cell fate control of pluripotent callus.
Ogura N, Sasagawa Y, Ito T, Tameshige T, Kawai S, Sano M, Doll Y, Iwase A, Kawamura A, Suzuki T, Nikaido I, Sugimoto K, Ikeuchi M
Science Advances 9: eadg698, 2023. https://www.science.org/doi/10.1126/sciadv.adg6983

•CRISPR/Cas9-based generation of mlo mutants for allelic complementation experiments to elucidate MLO function in barley.
Koide H, Hisano H, Yaeno T
Journal of General Plant Pathology 89:153-158. 2023. doi:10.1007/s10327-023-01120-w

• Optimizing genome editing efficiency in wheat: Effects of heat treatments and different promoters for single guide RNA expression.
Kishi-Kaboshi M, Abe F, Kamiya Y, Kawaura K, Hisano H & Sato K
Plant Biotechnology 40, 237-245. 2023. https://doi.org/10.5511/plantbiotechnology.23.0717a

• The HKT1 Na+ transporter protects plant fertility by decreasing Na+ content in stamen filaments.
Uchiyama T, Saito S, Yamanashi T, Kato M, Takebayashi K, Hamamoto S, Tsujii M, Takagi T, Nagata N, Ikeda H, Kikunaga H, Suda T, Toyama S, Miwa M, Matsuyama S, Seo M, Horie T, Kuromori T, Yamagami M, Ishimaru Y, Uozumi N
Science Advances 9: 22. 2023. https://doi.org/10.1126/sciadv.adg5495
 
• FLOURY ENDOSPERM 6 mutations enhance the sugary phenotype caused by the loss of ISOAMYLASE1 in barley.
Matsushima R, Hisano H, Galis I, Miura S, Crofts N, Takenaka Y, Oitome NF, Ishimizu T, Fujita N & Sato K
Theoretical and Applied Genetics 136:94. 2023. https://doi.org/10.1007/s00122-023-04339-5 

• Aerial roots of the leafless epiphytic orchid Taeniophyllum are specialized for performing crassulacean acid metabolism photosynthesis.
Suetsugu K, Sugita R, Yoshihara A, Okada H, Akita K, Nagata N, Tanoi K, Kobayashi K
New Phytologist 238(3): 932-937, 2023. https://doi.org/10.1111/nph.18812

• Discovery and Genome Characterization of a Closterovirus from Wheat Plants with Yellowing Leaf Symptoms in Japan.
Kondo H, Sugahara H, Fujita M, Hyodo K, Andika IB, Hisano H, Suzuki N
Pathogens 12(3): 358. 2023. https://doi.org/10.3390/pathogens12030358

• A bifurcated palea mutant infers functional differentiation of WOX3 genes in flower and leaf morphogenesis of barley.
Yoshikawa T, Hisano H, Hibara K-I, Nie J, Tanaka Y, Itoh J-I, Taketa S
AoB PLANTS 14(3). 2022. https://doi.org/10.1093/aobpla/plac019

• High-resolution map of plastid-encoded RNA polymerase binding patterns demonstrates a major role of transcription in chloroplast gene expression.
Palomar VM, Jaksich S, Fujii S, Kuciński J, Wierzbicki AT
Plant J. 2022 Aug;111(4):1139-1151. https://doi.org/10.1111/tpj.15882